Fig. 1 Linearity of the HISCL-5000 immunoassay for PSA. The red line and green lines indicate regression lines with linear and non-linear fit, respectively. Linearity was measured from 0.002 to 189.900 ng/mL.

Fig. 2 Method comparison for HISCL-5000 immunoassay and UniCel DxI-800 immunoassay for PSA (N=363, R2=0.99). (A) Measured range was from 0.01 to 541.56 ng/mL. The slope (95% confidence interval) was 0.92 (0.91-0.93) for PSA. The intercept (95% confidence interval) was -1.06 (-1.71~-0.40). (B) Bland-Altman plot shows the mean difference and upper and lower limit of 1.96 SD.

Fig. 3 Comparison of AUROCs in the BPH group with control group of non-malignant disease (A). PCa group with control group of non-malignant disease (B). BPH group with control group of healthy population (C). PCa group with control group of healthy population (D). Abbreviations: BPH, benign prostate hyperplasia; PCa, prostate cancer.